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CUT&RUN analysis of H3K9me1 and H3K27me3 in fibroblasts treated with TNFa ± TPCA1

ABSTRACT: Analysis of changes in chromatin modifications H3K9me1 and H3K27me3 in fibroblast cell line GM02036 (Coriell) after treatment with TNFa and/or TPCA1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE277146 | GEO | 2026/03/16

REPOSITORIES: GEO

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CUT&RUN analysis of H3K27ac and H3K4me1 in fibroblasts treated with TNFa ± deletion of RelA binding site on Chr21.

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PolyA RNA-seq from human fibroblast and retinal pigment epithelium cells treated with poly(I:C) or dsDNA

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H3K9me1.S2

Project description:modENCODE_submission_3770 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 5; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody H3K9me1 (target is H3K9me1)

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