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(6-4)PP and CPD Damage-seq data for L1 stage wildtype, csb-1 and xpc-1 C.elegans

ABSTRACT: Caenorhabditis elegans is widely used to study DNA repair and related processes such as aging, yet genome-wide nucleotide excision repair remains largely unexplored. Here, we present a comprehensive whole-organism map of UV-induced DNA damage and repair in L1 larvae. Of the two repair pathways - global repair and transcription-coupled repair - global repair predominates, accounting for over 80% of lesion removal; and notably, (6-4) photoproducts are removed by transcription-coupled repair at an extent comparable to CPD, a feature not previously observed in animals. Integration of damage and repair profiles with chromatin features reveals that despite non-uniform damage formation, repair efficiency is the stronger determinant of residual damage. Finally, repair around accessible regions exhibit periodicity, reflecting underlying nucleosome architecture. Together, these findings establish Caenorhabditis elegans as a valuable model organism for interrogating excision repair within a chromatin context and reveal species-specific features that broaden our understanding of DNA repair mechanisms across metazoans.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE280347 | GEO | 2025/09/19

REPOSITORIES: GEO

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