Distince macrophage subpopulations in mouse wound healing

ABSTRACT: Monocytes and macrophages displaying distinctive phenotypes may be pivotal in coordinating the transition between different stages of wound healing. RNAseq results showed that day 1 macrophages segregated from day 4 and 7 macrophages, and that on days 4 and 7 macrophages segregated primarily based on F4/80-low versus F4/80-high phenotype. TNF and IL-4 pathways converge in F4/80-low macrophages at 4 days after skin wound healing, these F4/80-low macrophages express inflammatory, MHC class II and a subset of IL-4 target genes. Footprinting analysis on ATAC-seq data revealed enrichement of both NFKB1 and STAT6 in F4/80-low macrophages at 4 dp, whereas the overall differences in transcription factor binding between F4/80-high and F4/80-low macrophages diminished by 7 dpi.

ORGANISM(S): Mus musculus

PROVIDER: GSE287605 | GEO | 2026/09/02

REPOSITORIES: GEO