Transcriptomics,Genomics

Dataset Information

40

Molecular mechanism for switching of P.falciparum invasion pathways into human erythrocytes


ABSTRACT: The malaria parasite, Plasmodium falciparum, exploits multiple ligand-receptor interactions, called invasion pathways, to invade the host erythrocyte. Strains of P.falciparum vary in their dependency on sialated red cell receptors for invasion. We show that switching from sialic acid-dependent to –independent invasion is reversible and depends on parasite ligand utilisation. Expression of P.falciparum reticulocyte-binding like homologue 4 (PfRh4) correlates with sialic acid-independent invasion and PfRh4 is essential for switching invasion pathways. Differential activation of PfRh4 represents a novel mechanism of invasion pathway control and provides P.falciparum with exquisite adaptability in the face of erythrocyte receptor polymorphisms and host immune responses. Keywords: Plasmodium falciparum, erythrocyte invasion, invasion pathways, EBA175, Rh4 Overall design: Experiment contains no replication. There are three genotypes: wildtype W2mef parasites, a cloned variant that can invade via a sialic acid independent pathway, and a parasite in which EBA175 has been knocked out and which also invades via a sialic acid independent pathway.

INSTRUMENT(S): Scripps Malaria GeneChip

SUBMITTER: Ken Simpson  

PROVIDER: GSE2878 | GEO | 2005-10-04

SECONDARY ACCESSION(S): PRJNA92557

REPOSITORIES: GEO

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Publications

Molecular mechanism for switching of P. falciparum invasion pathways into human erythrocytes.

Stubbs Janine J   Simpson Ken M KM   Triglia Tony T   Plouffe David D   Tonkin Christopher J CJ   Duraisingh Manoj T MT   Maier Alexander G AG   Winzeler Elizabeth A EA   Cowman Alan F AF  

Science (New York, N.Y.) 20050801 5739


The malaria parasite, Plasmodium falciparum, exploits multiple ligand-receptor interactions, called invasion pathways, to invade the host erythrocyte. Strains of P. falciparum vary in their dependency on sialated red cell receptors for invasion. We show that switching from sialic acid-dependent to -independent invasion is reversible and depends on parasite ligand use. Expression of P. falciparum reticulocyte-binding like homolog 4 (PfRh4) correlates with sialic acid-independent invasion, and PfR  ...[more]

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