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Mouse embryoid body differentiation in Profilin-2 3'UTR cis-element mutants

ABSTRACT: The coordinated actions of post-transcriptional regulation are essential for stem cell differentiation. The Profilin-2 transcript can be targeted by microRNAs and RNA binding proteins, specifically Ago2 and ESCC family of miRNAs -- miR-291-3p/294-3p/295-3p/302-3p -- and the Iron Regulatory Proteins IRP1/2. Earlier studies described the role of the conserved Profilin-2 ESCC miRNA site. This study describes the role of the conserved Profilin-2 IRP/iron-response-element site by CRISPR-mediated disruption of the Profilin-2 3'UTR iron response element site. The transcriptomic data reported indicates impaired germ layer formation and distribution during embryoid body differentiation when compared to wild-type mouse embryonic stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE288017 | GEO | 2026/08/18

REPOSITORIES: GEO

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