Project description:Proteome characterization of mesenchymal stem cells (MSC) and exosomes.
MSCs were cultured in normoxic, hypoxic and in presence of FBS. Exosomes were prepared from normoxic and hypoxic conditions.
Project description:Rat lung-resident mesenchymal stem cells (LR-MSCs) were isolated via bronchoalveolar lavage from fibroblast growth factor-10 (FGF-10) pretreated lungs. We characterized the similarity and diversity between LR-MSCs and bone marrow-derived mesenchymal stem cells (BM-MSCs) by transcriptional profiling of these two types of cells. In this dataset, we include the expression data obtained from LR-MSCs and BM-MSCs cultured under similar conditions at passage 5. These data are used to obtain genes that are differentially expressed by these two types of cells. 6 Total samples were analyzed. Both LR-MSCs and BM-MSCs are in three replicates.
Project description:Transcriptional profiling of liver cancer associated mesenchymal stem cells comparing normal mesenchymal stem cells from adjacent tumor-free tissues of the same patient 8, Goal was to determine to detect the paracrine trophic factors from liver cancer mesenchymal stem cells. 8 represents liver cancer associated MSCs, N8 represents liver normal MSCs Two-condition experiment, 8 vs. N8 cells. Biological replicates: 1 replicate from the same patient.
Project description:Transcriptional profiling of liver cancer associated mesenchymal stem cells comparing normal mesenchymal stem cells from adjacent tumor-free tissues of the same patient 8, Goal was to determine to detect the paracrine trophic factors from liver cancer mesenchymal stem cells. 8 represents liver cancer associated MSCs, N8 represents liver normal MSCs
Project description:Mesenchymal stem cells (MSCs) can modulate various immune responses implicated in the pathogenesis of sepsis. Intravenous injection of lipopolysaccharide (LPS) into healthy subjects represents a model with relevance for the host response to sepsis.
Project description:The composition and stiffness of the extracellular matrix (ECM) environment that surrounds Multipotent mesenchymal stem cells (MSCs) stem cells dictates transcriptional programming, thereby affecting stem cell lineage decision-making. Cells sense force between themselves and their microenvironment controlling the capability of MSCs to differentiate into adipocytes, osteocytes and chondrocytes. Force sensing is transmitted by integrin receptors and their associated adhesion signalling complexes. To identify regulators of MSC force sensing we sought to catalogue MSC adhesion complex composition. Therefore we isolated integrin-associated adhesion complexes formed in MSCs plated on the ECM ligand fibronectin. We identifed proteins using mass spectrometry that define a MSC specific subset of adhesion complex proteins consisting of key linkages to the actin cytoskeleton together with integrin signalling and force sensing components.
Project description:Rat lung-resident mesenchymal stem cells (LR-MSCs) were isolated via bronchoalveolar lavage from fibroblast growth factor-10 (FGF-10) pretreated lungs. We characterized the similarity and diversity between LR-MSCs and bone marrow-derived mesenchymal stem cells (BM-MSCs) by transcriptional profiling of these two types of cells. In this dataset, we include the expression data obtained from LR-MSCs and BM-MSCs cultured under similar conditions at passage 5. These data are used to obtain genes that are differentially expressed by these two types of cells.
Project description:The composition and stiffness of the extracellular matrix (ECM) environment that surrounds Multipotent mesenchymal stem cells (MSCs) stem cells dictates transcriptional programming, thereby affecting stem cell lineage decision-making. Cells sense force between themselves and their microenvironment controlling the capability of MSCs to differentiate into adipocytes, osteocytes and chondrocytes. Force sensing is transmitted by integrin receptors and their associated adhesion signalling complexes. To identify regulators of MSC force sensing we sought to catalogue MSC adhesion complex composition. Therefore we isolated integrin-associated adhesion complexes formed in MSCs plated on the ECM ligand fibronectin. We identifed proteins using mass spectrometry that define a MSC specific subset of adhesion complex proteins consisting of key linkages to the actin cytoskeleton together with integrin signalling and force sensing components.
Project description:Therapeutic benefits of mesenchymal stem/stromal cells (MSCs) are now widely believed to come from their paracrine signalling, i.e. secreted factors such as cytokines, chemokines, and extracellular vesicles (EVs). Cell-free therapy using EVs is an active and emerging field in regenerative medicine. The cellular environment of MSCs is of critical importance when directing paracrine activity. Typical 2D cultivation of stem cells on tissue culture plastic is far removed from the physiological environment of MSCs. The application of 3D cell culture allows MSCs to adapt to their cellular niche environment which, in turn, influences their paracrine signalling activity. In this study we evaluated the impact of 3D MSCs culture on EVs secretion and cargo proteome composition and functional assessment. The outcome highlights critical differences between MSC-EVs obtained from different culture microenvironments, which should be considered when scaling up MSC culture for clinical manufacturing.