Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing analysis of the global expression profiling in bone marrow-derived macrophages (BMDMs) from wild-type and RBP4-deficient mice.

ABSTRACT: To investigate the influence of retinol-binding protein 4 (RBP4) deficiency on the expression of genes related to cholesterol metabolism, we examined the expression profiling of global genes in BMDMs from wild-type(WT) and RBP4-deficient mice (RBP4-KO) by RNA-seq. The results revealed that expression of 71 genes were greater than twofold in RBP4-KO BMDMs relative to their expression levels in WT cells.In addition, the expression of 158 genes were lower than in RBP4-KO BMDMs compared to that of WT cells. Clustering analysis suggested that deficiency of RBP4 suppressed the expression of a subset of cholesterol uptake-associated genes without affecting the expression of genes related to cholesterol synthesis or efflux, indicating that RBP4 is likely involved in regulating cholesterol homeostasis.

ORGANISM(S): Mus musculus

PROVIDER: GSE290075 | GEO | 2025/10/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Proteomic analysis of bone-marrow derived macrophages from NPC1 KO mice

Project description:Niemann-Pick type C (NPC) disease is an inherited lysosomal storage disorder mainly driven by mutations in NPC1 gene, causing lipid accumulation within late endosomes/lysosomes, and resulting in progressive neurodegeneration. To study the effect of NPC1 deficiency on the innate immune system, we performed proteomics on bone-marrow derived macrophages (BMDMs) of NPC1 KO and WT mice. Without further treatment or activation, BMDMs of NPC1 KO mice showed alterations mainly related to cholesterol metabolism, which is in line with the intracellular cholesterol transport function of NPC1.

2024-12-05 | PXD056249 | Pride

Gene expression from bone-marrow derived macrophages.

Project description:Gene expression from WT and NFAT5 KO primary macrophage cultures. Keywords: Bone-marrow derived macrophages. We analyzed 4 arrays from each condition: unstimulated WT BMDMs, LPS stimulated WT BMDMs, unstimulated KO BMDMs, LPS stimulated KO BMDMs.

2012-01-20 | E-GEOD-26343 | biostudies-arrayexpress

mRNA-seq to test effect of PYK2 inhibitor (defactinib) on LPS induced gene expression in WT and IRF5-/- BMDMs

Project description:To investigate the specificity of Defactinib on IRF5 target genes we compare LPS-induced transcriptomes in WT and IRF5 KO BMDMs treated with either Defactinib or vehicle. Macrophage transcriptomic signature under PYK2 inhibition phenocopied IRF5 deficiency

2021-10-22 | GSE141082 | GEO

Effects of Irg1-itaconate or Hemin on gene expression in macrophages [RNA-seq]

Project description:To investigate the role of Irg1-itaconate or Hemin in macrophages, bone marrow-derived macrophages (BMDMs) from WT mice and Irg1 KO mice were extracted and cultured. After 7 days of cells culture, the BMDMs from WT mice were treated 120 μM 4-octyl itaconate (4-OI) for 15 h, 15 μM Hemin for 12 h, and the BMDMs from Irg1 KO mice and WT mice were treated Vehicle for 12 h. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4-OI, Hemin or vehicle treated WT BMDMs and Irg1 KO BMDMs.

2024-06-28 | GSE221734 | GEO

RNA-seq analysis of Wild Type (WT) and Mettl3-/- (KO) BMDMs (bone-marrow-derived macrophages) Transcriptomes

Project description:To investigate the role of METTL3-mediated m6A modification in macrophage, we performed RNA-sequencing in bone-marrow-derived macrophages (BMDMs) in wild type (WT) and Mettl3-/- (KO) mice We then performed gene expression profiling analysis using data obtained from RNA-seq of WT and KO BMDMs

2025-11-04 | GSE217269 | GEO

Transcript profiling of WT and SIRPα KO BMDMs post-M1 activation in the presence of mCD47.ex

Project description:To compare the inflammatory responses of WT and SIRPα KO macrophage, we performed a complete transcript profiling of WT and SIRPα-KO M1 macrophage using transcriptome sequencing as a discovery platform. SIRPα-KO mice and WT mice were kept under the same condition. BMDMs were produced from WT and SIRPα-KO mice followed by M1 polarization. RNA was then isolated from the same number of BMDMs.

2021-03-19 | GSE169177 | GEO

Setd2 reprograns lipid metabolism for the resolution of inflammation II

Project description:Purpose: The goal of this study is to identify differential expression profiles between WT and Setd2-KO in BMDMs induced by LPS for a time-course stimulaiton. Methods: We profiled RNA-seq in bone marrow-derived macrophages stimulated with LPS for 0h, 12h, 24h. Results: We find significant up and down regulated genes in Setd2-KO BMDMs under LPS stimulation.

2022-11-13 | GSE141474 | GEO

The proteomic of IL-4 treated BMDMs from Wt and Elp3 KO mice

Project description:This experiement aims to know what the differences of protein translation are in the bone marrow derived macro-phages(BMDMs) from WT mice and Elp3 KO mice. We treated the BMDMs with or without IL-4 for 4 hours.

2022-09-12 | PXD033962 | Pride

ChIP-seq to profile LPS-induced RNA polymerase II transcription in bone marrow-derived macrophages from WT and Hes1 KO mice

Project description:We characterized the RNA polymerase II occupancy on gene loci in WT and Hes1 KO BMDMs under untreated and LPS-stimulated conditions WT and Hes1 KO BMDMs were left untreated or stimulated with LPS for 1 hour. Pol II ChIP was performed and the DNA products were subject to ChIPseq

2016-06-13 | E-GEOD-73568 | biostudies-arrayexpress

Genome-wide maps of chromatin state and RNA-seq in WT and Dnmt3a KO BMDMs (bone marrow derived macrophages) [RRBS, ATAC-seq, CHIP-seq]

Project description:To identify soluble factors released from Dnmt3a KO macrophages that might drive differences in osteoclast differentiation, we performed RNA sequencing on unstimulated BMDMs from WT and Dnmt3a KO mice. To investigate the mechanistic basis for the inflammatory phenotype. of myeloid cells with loss of Dnmt3a,we performed reduced representation bisulfite sequencing (RRBS) on Dnmt3a KO and WT BMDMs to assess methylation changes. Concurrently, we performed ATAC-sequencing (ATAC-seq) to assess differences in chromatin accessibility between WT and Dnmt3a KO BMDMs in the context of changes in methylation. We performed chromatin immunoprecipitation sequencing (CHIP-seq) for Irf3 and Rela based on ATAC-seq analysis.

2021-10-07 | GSE184443 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data