Dataset Information

Comparative Transcriptome Analyses Reveal the Responses to Hypoxia Challenge of Mactra veneriformis

ABSTRACT: The transcriptomic analysis of Mactra veneriformis under hypoxia stress was carried out in this paper to explore the response mechanism of M. veneriformis under hypoxia stress from the molecular biological level. The results provided a reference for further study on hypoxia tolerance mechanism and creation of new hypoxia tolerant germplasm of Clams quadricornis. The findings suggest that transcriptome sequencing was performed on M. veneriformis branchial filament tissues under normal and hypoxia conditions by high-throughput sequencing technology, and 54.58Gb of clean data were obtained. There were differences in gene expression between the normal-oxygen group and the hypoxia group. Control group A and hypoxia group B had the most differentially expressed genes (7473 genes), among which 4000 up-regulated genes and 3473 down-regulated genes. Secondly, there were 6407 differentially expressed genes between hypoxia group B and hypoxia group C, with 3535 up-regulated genes and 2872 down-regulated genes. There were 5581 differentially expressed genes in the control group A and the hypoxia group C, 2676 up-regulated genes and 2905 down-regulated genes. According to the enrichment of GO functional annotations, differential genes were mainly concentrated in the membrane of cell components, metabolism in biological processes and binding of molecular functions. The enrichment of KEGG pathway showed that multiple metabolic and genetic information processing pathways were activated.

ORGANISM(S): Mactra quadrangularis

PROVIDER: GSE293462 | GEO | 2025/04/06

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:In order to study the gene expression profile in C57Bl/10 mouse blood, we exposed three different groups of animals. First was exposed to PO2 21% or normoxia. The second was exposed to chronic hypoxia (from PO2 21% to PO2 8%) and the third was also exposed to the same chronic hypoxia (CH) protocol but followed by two weeks under normoxia, and called as recovery group. The blood was extracted from inferior vena cava, the RNA was extracted, amplified and hybridized to Affimetrix MOE 430 V2.o chip. The results were analyzed using Partek Genome suite software. Using two fold cuttoff and 0% FDR parameters, we observed genes 512 diferentially expressed, of which one gene was up-regulated in both hypoxic and recovery condition, 202 were up-regulated during CH and then down-regulated after the recovery, 18 genes were down-regulated afteh CH and the up-regulated after recovery, ans finally 9 genes were down-regulated in both CH and recovery conditions. Each group of C57Bl/10 mouse had 4 individuals and they were divided into three different groups of animals. First was exposed to PO2 21% or normoxia for 14 days. The second was exposed to chronic hypoxia (from PO2 21% to PO2 8%) and the third was also exposed to the same chronic hypoxia (CH) protocol but followed by two weeks under normoxia, and called as recovery group. After the exposure period, the animals were anaesthetized with a mix of Ketamine (100 mg/mL) and xylasine (20 mg/mL) in a proportion of 1:1. A incison was made on abdominal area and from kidneis and liver area of inferior vena cava venus blood was collected The blood was extracted from inferior vena cava. Then, the RNA was extracted, amplified and hybridized to Affimetrix MOE 430 V2.o chip. The results were analyzed using Partek Genome suite software. Using two fold cuttoff and 0% FDR parameters, we observed genes 512 diferentially expressed, of which one gene was up-regulated in both hypoxic and recovery condition, 202 were up-regulated during CH and then down-regulated after the recovery, 18 genes were down-regulated afteh CH and the up-regulated after recovery, ans finally 9 genes were down-regulated in both CH and recovery conditions.

Dataset Information

Comparative Transcriptome Analyses Reveal the Responses to Hypoxia Challenge of Mactra veneriformis

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets