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A Silk-Based Patch with Temporary Adhesion and Detachability on the Ocular Surface for Inflammatory Mediator Removal

ABSTRACT: Corneal alkali burns cause persistent inflammation, leading to corneal vascularization and fibrosis, which severely impair vision. Here, we developed a temporary adhesive silk-based patch to capture and remove inflammatory mediators from the ocular surface. The patch utilizes photocrosslinkable silk for mechanical support, chitosan as the adhesive, hyaluronan for lubrication, and polyamidoamine (PAMAM) dendrimers with heparin to enhance adsorption. After water annealing, the patch exhibited excellent transparency, mechanical strength, and resistance to swelling, remaining attached to the rat ocular surface for 3–5 days. Adsorption tests confirmed that the patch effectively captured small-molecule dyes, proteins, and free DNA. In the rat corneal alkali burn model, imaging and histological evaluations showed significant reductions in vascularization and fibrosis after 3 days of treatment, along with improved corneal transparency. RNA sequencing revealed that patch treatment effectively inhibited the PI3K–AKT inflammatory pathway. This inflammation-removing patch represents an innovative treatment for corneal alkali burns with significant clinical potential.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE295198 | GEO | 2025/06/25

REPOSITORIES: GEO

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Exploring Corneal Neovascularization: An Integrated Approach Using Transcriptomics and Proteomics in an Alkali Burn Mouse Model

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