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Small RNA sequencing of adipose tissue derived extracellular vesicles from chow- or HFD-fed male mice

ABSTRACT: We identified EVs as novel regulatory factors that mediate adipose tissue–brain communication and are crucial for the central control of whole-body energy homeostasis. Our findings reveal that AT-EVs and their cargo microRNAs (miRNAs) are crucial regulators of central leptin sensitivity. To identify the specific subtypes of miRNAs that responsible for central leptin-sensitizing, we examined the sRNA profiles of EVs extracted from adipose tissue of healthy chow-fed and high-fat diet mice by RNA deep sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE295694 | GEO | 2026/04/10

REPOSITORIES: GEO

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Small RNA sequencing of extracellular vesicles extracted from adipose tissue of healthy weight or obese human subjects

Project description:We identified EVs as novel regulatory factors that mediate adipose tissue–brain communication and are crucial for the central control of whole-body energy homeostasis. Our findings reveal that AT-EVs and their cargo microRNAs (miRNAs) are crucial regulators of central leptin sensitivity. To identify the specific subtypes of miRNAs that responsible for central leptin-sensitizing, we examined the sRNA profiles of EVs extracted from adipose tissue of healthy weight and obese subjects by RNA deep sequencing.

2026-04-10 | GSE295693 | GEO

Small RNA sequencing of adipose tissue derived extracellular vesicles and hippocampus from chow- or HFD-fed male mice

Project description:We demonstrated that adipose tissue derived extracellular vesicles from HFD-fed mice could induce cognitive impairment. miRNAs in adipose tissue-derived EVs could be transferred to the hippocampus, which promotes synaptic damage and cognitive impairment. To identify the specific subtypes of miRNAs that responsible for cognitive impairment, we examined the sRNA profiles of hippocampus and EVs extracted from adipose tissue of Chow and HFD mice by RNA deep sequencing.

2025-05-01 | GSE203398 | GEO

Leptin-Activated Crosstalk Among Brain, Brown Adipose Tissue, and Heart Drives Cardiac Protection After Ischemia-Reperfusion Injury [miRNA-seq]

Project description:Cardiovascular disease remains the leading cause of death worldwide, with coronary artery disease being the primary contributor. Our recent studies suggest that activation of leptin receptors (LepRs) in the brain can improve cardiac function following myocardial infarction. However, the mechanism by which this cardioprotective effect is transmitted from the brain to the heart remains unclear. We hypothesize that brain LepR activation stimulates brown adipose tissue (BAT) to secrete extracellular vesicles (EVs) enriched with cardioprotective factors. These EVs may safeguard the heart by modulating cardiac mitochondrial function and collagen deposition. Our findings indicate that BAT ablation or BAT sympathetic denervation diminishes the cardioprotective effects of brain LepR activation. We also observed an increased concentration of EVs within BAT of rats treated with ICV leptin compared to vehicle-treated controls, an effect abolished by BAT denervation. Furthermore, knockdown of Rab27a in BAT reduced the cardioprotective benefits of brain LepR activation. MicroRNA (miR)-29c-3p was identified as a cargo of leptin-stimulated BAT-derived EVs and appears to play a key role in mitigating cardiac fibrosis after IR injury in leptin-treated animals. Thus, activation of LepR in the brain protects the heart after IR injury via sympathetic mediated BAT-derived EVs enriched with miR-29c-3p.

2026-01-05 | GSE315349 | GEO

Leptin-Activated Crosstalk Among Brain, Brown Adipose Tissue, and Heart Drives Cardiac Protection After Ischemia-Reperfusion Injury [RNA-seq]

2026-01-05 | GSE315348 | GEO

Effect of long-term education with EVs from women with obesity on MCF7 gene expression

Project description:Breast adipose tissue is an important contributor to the obesity-breast cancer link. Dysregulated cell metabolism is now an accepted hallmark of cancer. Extracellular vesicles (EVs) are nanosized particles containing selective cargo, such as miRNAs, that act locally or circulate to distant sites to modulate target cell functions. Here, we found that long-term education of breast cancer cells (MCF7, T47D) with EVs obtained from breast adipose tissue of women who are overweight or obese (O-EVs) leads to sustained increased proliferative potential. RNA-Seq of O-EV-educated MCF7 cells demonstrates increased expression of genes, such as ATP synthase and NADH: ubiquinone oxidoreductase, involved in oxidative phosphorylation.

2023-10-31 | GSE245776 | GEO

Integrated transcriptomic and proteomic analysis of the molecular cargo of extracellular vesicles derived from porcine adipose tissue-derived mesenchymal stromal/stem cells

Project description:Background: Mesenchymal stromal/stem cell (MSC) transplantation is a promising therapy for tissue regeneration. Extracellular vesicles (EVs) released by MSCs act as their paracrine effectors by delivering proteins and genetic material to recipient cells. To assess how their cargo mediates biological processes that drive their therapeutic effects, we integrated miRNA, mRNA, and protein expression data of EVs from porcine adipose tissue-derived MSCs. Methods: Simultaneous expression profiles of miRNAs, mRNAs, and proteins were obtained by high-throughput sequencing and LC-MS/MS proteomic analysis in porcine MSCs and their daughter EVs (n=3 each). TargetScan and ComiR were used to predict miRNA target genes. Functional annotation analysis was performed using DAVID 6.7 database to rank primary gene ontology categories for the enriched mRNAs, miRNA target genes, and proteins. STRING was used to predict associations between mRNA and miRNA target genes. Results: Differential expression analysis revealed 4 miRNAs, 255 mRNAs, and 277 proteins enriched in EVs versus MSCs (fold change >2, p<0.05). EV-enriched miRNAs target transcription factors (TFs) and EV-enriched mRNAs encode TFs, but TF proteins are not enriched in EVs. Rather, EVs are enriched for proteins that support extracellular matrix remodeling, blood coagulation, inflammation, and angiogenesis. Conclusions: Porcine MSC-derived EVs contain a genetic cargo of miRNAs and mRNAs that collectively control TF activity in EVs and recipient cells, as well as proteins capable of modulating cellular pathways linked to tissue repair. These properties provide the fundamental basis for considering therapeutic use of EVs in tissue regeneration.

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Osteoblasts secrete miRNA-containing extracellular vesicles that enhance expansion of human umbilical cord blood cells

Project description:Osteolineage cells represent one of the critical bone marrow niche components that support maintenance of hematopoietic stem and progenitor cells (HSPCs). Recent studies demonstrate that extracellular vesicles (EVs) regulate stem cell development via horizontal transfer of bioactive cargo, including microRNAs (miRNAs). Here, we characterize the miRNA profile of EVs secreted by human osteoblasts and study their biological effect of on human umbilical cord blood-derived CD34+ HSPCs by sequencing, gene expression and biochemical analyses. Using next-generation sequencing we show that osteoblast-derived EVs contain highly abundant miRNAs specifically enriched in EVs, including critical regulators of hematopoietic proliferation (e.g., miR-29a). EV treatment of CD34+ HSPCs alters the expression of candidate miRNA targets, such as HBP1, BCL2 and PTEN. Furthermore, EVs enhance proliferation of CD34+ cells and their immature subsets in growth factor-driven ex vivo expansion cultures. Importantly, EV-expanded cells retain their differentiation capacity in vitro and show successful engraftment in NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice in vivo. These discoveries reveal a novel osteoblast-derived EV-mediated mechanism for regulation of HSPC proliferation and warrant consideration of EV-miRNAs for the development of expansion strategies to treat hematological disorders.

2016-10-05 | E-MTAB-4560 | biostudies-arrayexpress

miRNA expression profiling of visceral adipose tissue-derived extracellular vesicles in nicotine-treated ApoE-/- mice

Project description:This study profiles the miRNA expression of visceral adipose tissue-derived extracellular vesicles (VAT-EVs) in ApoE-deficient (ApoE-/-) mice subjected to different dietary and treatment conditions. Mice were fed either a high-fat diet (HFD) or a high-fat diet combined with nicotine administration (HFD+Ni). Epididymal white adipose tissue (eWAT) was used as a representative visceral fat depot for EV isolation. High-throughput small RNA sequencing was performed to characterize the miRNA cargo of VAT-EVs and to identify differentially expressed miRNAs between the two groups. The resulting dataset provides a resource for investigating nicotine-associated alterations in EV-mediated miRNA signaling in the context of metabolic and inflammatory processes.

2026-03-25 | GSE314694 | GEO

Characterization and miRNA Profiling of Extracellular Vesicles from Rabbit Oviduct and Uterine Fluids

Project description:This study aims to uncover how extracellular vesicles (EVs) influence key reproductive processes by isolating, characterizing, and analyzing the miRNA cargo of these oviductal and uterine fluid-EVs. Small RNA sequencing identified 388 miRNAs in OF-EVs and UF-EVs. However, 4 miRNAs significantly more abundant in OF-EVs, while 17 were significantly more abundant in UF-EVs, indicating potential differences in their roles in embryo-maternal communication. The most abundant miRNAs in both fluids included ocu-miR-148a-3p, ocu-let-7i-5p, and ocu-miR-10b-5p, which are associated with embryo development and implantation. Gene target prediction analysis of the top abundant revealed that miRNAs in OF-EVs regulate key signaling pathways such as MAPK, Hippo, TGF-β, Ras, PI3K-Akt, and mTOR, while UF-EVs are associated with Hippo, PI3K-Akt, FoxO, endocytosis, and mTOR signaling. These pathways are essential for cell proliferation, differentiation, and immune regulation, crucial for embryo-maternal interactions. Our findings suggest that EVs in rabbit reproductive fluids play a fundamental role in early embryonic development and successful pregnancy. These results provide new insights into reproductive biotechnology and veterinary medicine, with potential applications in improving fertility and embryo viability.

2026-02-02 | GSE297220 | GEO

Extracellular vesicles isolated from human induced pluripotent stem cell-derived neurons contain a transcriptional network

Project description:Healthy brain function is mediated by several complementary signalling pathways, many of which are driven by extracellular vesicles (EVs). EVs are heterogeneous in both size and cargo and are constitutively released from cells into the extracellular milieu. They are subsequently trafficked to recipient cells, whereupon their entry can modify the cellular phenotype. Here, in order to further analyse the mRNA and protein cargo of neuronal EVs, we isolated EVs by size exclusion chromatography from human induced pluripotent stem cell (iPSC)-derived neurons. Electron microscopy and dynamic light scattering revealed that the isolated EVs had a diameter of 30-100 nm. Transcriptomic and proteomics analyses of the EVs and neurons identified key molecules enriched in the EVs involved in cell surface interaction (integrins and collagens), internalisation pathways (clathrin- and caveolin-dependent), downstream signalling pathways (phospholipases, integrin-linked kinase and MAPKs), and long-term impacts on cellular development and maintenance. Overall, we show that key signalling networks and mechanisms are enriched in EVs isolated from human iPSC-derived neurons.

2020-08-20 | E-MTAB-8254 | biostudies-arrayexpress

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