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Acclimation of the Escherichia coli transcriptome after 10 hours of incubation in the presence of copper sulfate at sublethal levels.

ABSTRACT: Copper sulfate has been explored as an FDA-approved alternative to treat antibiotic-resistant bacterial strains. Questions remain as it relates to the full-systems response elicited to counteract its toxic effects, especially in the context of a non-lethal exposure. We explored the transcriptomic response profile of Escherichia coli K12 BW25113 when challenged to grow planktonically for 10 hours in M9-glucose minimal media spiked with a sublethal concentration of copper sulfate (39 µM, 6.22 µg/mL). Our results yielded changes in gene expresson levels: 80 genes were up-regulated, while 76 were down-regulated in our copper sulfate treatment. Some biological processes became dysregulated to cope with the presence of this metal stressor, including iron metabolism, sulfur metabolism, cysteine biosynthesis, and the copper homeostasis systems Cue and Cus. Altogether, this work provides a valuable snapshot of how our indicator strain adapts to metal-induced stress. This is a significant step in understanding how bacteria can adjust their physiology to coexist with sublethal concentrations of metal-based antimicrobials.

ORGANISM(S): Escherichia coli

PROVIDER: GSE295970 | GEO | 2025/08/13

REPOSITORIES: GEO

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