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Conformational Dynamics in RNA Cleavage Specificity During Dicing

ABSTRACT: In this study, using massively parallel dicing assays and cryo-electron microscopy, we identify a previously unrecognized guanosine-favored (G-favored) binding pocket in DICER that exhibits a striking specificity for guanosine. This discovery fundamentally redefines the canonical view of DICER substrate recognition, as it contrasts with the known uridine-favored (U-favored) pocket, which binds uridine, adenine, and cytosine while excluding guanosine. We demonstrate that these distinct G- and U-favored pockets govern the generation of 21-nt and 22-nt small RNAs, respectively, revealing a new layer of control in small RNA biogenesis

ORGANISM(S): synthetic RNA

PROVIDER: GSE296721 | GEO | 2026/05/20

REPOSITORIES: GEO

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