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Effect of ASC-J9 on gene expression of murine bone marrow-derived macrophages

ABSTRACT: The Androgen receptor (AR) in prostate cancer cells acts as a central transcription factor, playing a crucial role in maintaining tumour cell survival and the production of prostate-specific antigen (PSA).To investigate the effect of AR on macrophage gene phenotype, mouse bone marrow-derived macrophages (BMDMs) treated with the AR-degrading agent ASC-J9 in presence of conditioned media (CM) derived from RM1 mouse prostate cancer cells (RM1 CM) was collected for RNA sequencing (RNA-seq). Differential expressed genes (DEGs) analysis showed that compared to DMSO-treated macrophages, ASC-J9-treated macrophages had lower expressions of Trem2 and Trem1, anti-inflammatory M2-like macrophage markers Cd163 and Arg1 expression, pro-migratory factors Ccl2 and Ccl8, but had higher expressions of pro-inflammatory M1-like macrophage markers Cd86 and Tnf, suggesting that AR may be associated with macrophage phenotypic differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE296857 | GEO | 2025/06/23

REPOSITORIES: GEO

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