Dataset Information

Removal of extracellular matrix from the cell surface improves adhesion of primary human hepatocytes

ABSTRACT: While investigating the causes of unstable adhesiveness of primary human hepatocytes, we observed that non-adhering cells had some kind of extracellular matrix on their surface, and this extracellular matrix inhibited cell adhesion to culture vessels. Removal of the matrix from the cell surface of non-adhering cells improved cellular attachment to culture vessels. We also performed comparative gene expression analysis between adhering and non-adhering cells to elucidate the molecular basis of differential adhesion behavior. We investigated the unstable adhesiveness of primary human hepatocytes and found that non-adhering cells had extracellular matrix on their surface that inhibited cell adhesion. We also observed that matrix removal improved cellular attachment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE299034 | GEO | 2025/12/17

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Multiple myeloma involves early dissemination of malignant plasma cells across the bone marrow; however, the initial steps of dissemination remain unclear. Human bone marrow-derived mesenchymal stromal cells (hMSCs) stimulate myeloma cell expansion (e.g., IL-6) and simultaneously retain myeloma cells via chemokines (e.g., CXCL12) and adhesion factors. Hence, we hypothesized that the imbalance between cell division and retention drives dissemination. We present an in vitro model using primary hMSCs co-cultured with INA-6 myeloma cells. Time-lapse microscopy revealed proliferation and attachment/detachment dynamics. Separation techniques (V-well adhesion assay and well plate sandwich centrifugation) were established to isolate MSC-interacting myeloma subpopulations that were characterized by RNAseq, cell viability and apoptosis. Results were correlated with gene expression data (n=837) and survival of myeloma patients (n=536). On dispersed hMSCs, INA-6 saturate hMSC-surface before proliferating into large homotypic aggregates, from which single cells detached completely. On confluent hMSCs, aggregates were replaced by strong heterotypic hMSC-INA-6 interactions, which modulated apoptosis time-dependently. Only INA-6 daughter cells (nMA-INA6) detached from hMSCs by cell division but sustained adherence to hMSC-adhering mother cells (MA-INA6). Isolated nMA-INA6 indicated hMSC-autonomy through superior viability after IL6 withdrawal and upregulation of proliferation-related genes. MA-INA6 upregulated adhesion and retention factors (CXCL12), that, intriguingly, were highly expressed in myeloma samples from patients with longer overall and progression-free survival, but their expression decreased in relapsed myeloma samples. Altogether, in vitro dissemination of INA-6 is driven by detaching daughter cells after a cycle of hMSC-(re)attachment and proliferation, involving adhesion factors that represent a bone marrow-retentive phenotype with potential clinical relevance.

Dataset Information

Removal of extracellular matrix from the cell surface improves adhesion of primary human hepatocytes

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets