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Removal of elastin from the cell surface of primary human hepatocytes

ABSTRACT: Primary human hepatocytes exhibit variable adhesiveness to culture vessels, which limits their utility in drug screening and regenerative medicine. We investigated the cause of this unstable adhesiveness of primary human hepatocytes and observed that the extracellular matrix on the surface inhibits cell adhesion to culture vessels. Elastin was identified as one of the main components of the surface extracellular matrix of non-adhering cells. This discovery suggests a novel approach to enhance their stable adhesion. Unstable adhesion of primary human hepatocytes to culture vessels significantly hinders their use in research. We discovered that extracellular matrix components on the cell surface inhibit their attachment. Specifically, we identified elastin as a key problematic component in non-adhering cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE299848 | GEO | 2025/12/17

REPOSITORIES: GEO

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