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NGLY1-dependent conversion of N-glycosylated N to D is essential for transcription of proteasome genes

ABSTRACT: To investigate the sequence editing-specific effects on Nrf1 functions, we utilized glycan-less Nrf1 mutants, 9NA and 9ND, in which all nine of the putative N-glycosylation sites (N-X-S/T; asparagine (N) serine (S), threonine (T), and X is any amino acid except proline) were replaced with alanine (A) and aspartic acid (D), respectively. To comprehensively understand the effects of Nrf1 sequence editing on the transcriptome, microarray analysis was performed on NGLY1-KO HeLa cells stably expressing 9NA and 9ND.

ORGANISM(S): Homo sapiens

PROVIDER: GSE301626 | GEO | 2025/12/14

REPOSITORIES: GEO

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NGLY1-dependent conversion of N-glycosylated N to D is essential for transcription of proteasome genes

Project description:NGLY1-dependent conversion of N-glycosylated N to D is essential for transcription of proteasome genes

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