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Posphorylation-Dependent Activation of Transcription Factors Drives Cell Fate Switching in Cortical Development

ABSTRACT: Cortical development involves precise regulation of transcription factors (TFs) and signalling pathways. Here, we show that phosphorylation of TFs is a key mechanism driving cell fate transitions during neurogenesis and gliogenesis. Using multi-omics profiling of embryonic mouse cortex, we identify stage-specific phosphorylation of Hmgn3 and Nfib as critical for neuronal differentiation and maturation. Phospho-mutant analyses reveal impaired migration and astrocyte differentiation. CUT&RUN and scATAC-seq integration show phosphorylation-dependent enhancer binding by Nfib. Additionally, Hmgn3 and Nfib regulate distinct signaling pathways mediating cell-cell communication. These findings highlight phosphorylation as a central regulator of TF activity and cortical lineage specification.

ORGANISM(S): Mus musculus

PROVIDER: GSE302501 | GEO | 2026/07/20

REPOSITORIES: GEO

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