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Comparative phenotypic characterisation of mutant human bronchial epithelial cells in vitro and in vivo

ABSTRACT: Development of tractable and robust models of cancer depends on a transparent, detailed and critical evaluation of their advantages and disadvantages for their use in basic and translational cancer research. Specifically, identifying the phenotypic differences of cancer cells in vivo and in vitro is essential to select the most informative experimental setting without compromising the scalability, versatility and cost-effectiveness of research projects. Development of tractable and robust models of cancer depends on a transparent, detailed and critical evaluation of their advantages and disadvantages for their use in basic and translational cancer research. Specifically, identifying the phenotypic differences of cancer cells in vivo and in vitro is essential to select the most informative experimental setting without compromising the scalability, versatility and cost-effectiveness of research projects. Our observations showed similar results in vivo and in vitro regarding the essential components for the transformation of hBECs. We also observe differences in gene expression that suggest an increase in motility in vivo and, importantly, an alternative mechanism of LUSC development that depends on squamous differentiation mediated by epithelial inactivation of NOTCH1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE302966 | GEO | 2026/07/14

REPOSITORIES: GEO

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