Single-cell RNA and TCR sequencing of tumor-infiltrating lymphocytes and draining lymph node immune cells following AMP-410 treatment

ABSTRACT: We employed single-cell RNA sequencing (scRNA-seq) and single-cell TCR sequencing (scTCR-seq) using the 10X Genomics platform to investigate immune cell dynamics during AMP-410 treatment in MC38 tumor-bearing b-h4-1BB transgenic mice. AMP-410 is a bifunctional VEGF-4-1BB biologic designed to simultaneously target VEGF and 4-1BB pathways, delivering a localized and synergistic approach to cancer immunotherapy. This study demonstrates that dual targeting of VEGF and 4-1BB signaling normalizes the tumor microenvironment, prevents T cell exhaustion in the TME in the later stage and also reduce the T cell conal exchange between tumor microenvironment and draining lymph nodes (dLNs), suggesting a local activity.

ORGANISM(S): Mus musculus

PROVIDER: GSE307999 | GEO | 2026/09/10

REPOSITORIES: GEO