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High-glucose environment impairs keratinocyte migration and proliferation through the ROS/Akt/ETV4 signaling pathway

ABSTRACT: The RNA-sequencing dataset comprises eight human immortalized keratinocyte samples cultured under high-glucose conditions. Cells were exposed to 35 mM glucose for 96 h and transfected with either ETV4-targeting small interfering RNA (siETV4) or negative-control small interfering RNA (siNC) for 48 h. Four independent biological replicates were included in each group. Normal-glucose and mannitol osmotic-control samples were used in separate cellular validation experiments but were not included in the RNA-sequencing dataset. Human immortalized keratinocytes within passages P21?P33 were maintained in customized RPMI-1640 medium containing 5.5 mM glucose (L210KJ, Yuanpei Biology, Shanghai, China), supplemented with 10% fetal bovine serum and 1% penicillin?streptomycin. For RNA sequencing, the glucose concentration was adjusted to 35 mM. Cells were exposed to high-glucose conditions for 96 h and transfected with siETV4 or siNC for 48 h.

ORGANISM(S): Homo sapiens

PROVIDER: GSE309206 | GEO | 2026/01/06

REPOSITORIES: GEO

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