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Single-nucleus RNA sequencing of male and female brain in a tau P301L transgenic zebrafish

ABSTRACT: The microtubule-associated protein tau forms pathological aggregates in Alzheimer’s disease and related tauopathies, and animal models are widely used to dissect the cellular processes involved. Transgenic zebrafish expressing human mutant tau provide a vertebrate system in which neuronal tau pathology can be examined, yet single-cell-resolution transcriptomic resources for such models remain scarce. Here we describe a single-nucleus RNA sequencing dataset generated from whole-brain tissue of six-month-old transgenic zebrafish expressing human tau carrying the P301L mutation under a neuron-specific promoter, alongside wild-type controls. Both sexes were profiled, with two replicate libraries per sex and genotype, giving eight libraries in total. Nuclei were isolated from pooled brains, processed with a droplet-based 3′ assay, and sequenced to generate transcriptome-wide expression profiles across the major brain cell types. We provide the raw sequencing reads, processed gene-by-nucleus count matrices, and cell-type annotations. This resource can be reused to study cell-type-specific and sex-resolved transcriptional features of the model and to support comparative analyses with mammalian datasets.

ORGANISM(S): Danio rerio

PROVIDER: GSE310345 | GEO | 2025/12/10

REPOSITORIES: GEO

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