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Identification of a novel deeply quiescent neural stem cell population in the subventricular zone

ABSTRACT: This study shows that a novel genetic tool, the Penk-CreAi9 line, expressing Cre recombinase under the Proenkephalin promoter and combined with the Ai9-tdTomato line, labels a distinct group of stem cells in the SVZ together with previously identified subgroups of B-cells and neuroblasts. Single-cell RNA sequencing (scRNA-seq) demonstrates that these cells represent a previously unappreciated population of qNSCs with a unique transcriptional profile and a less differentiated state than the canonically accepted GLAST+ NSCs. In particular, the analysis further reveals expression of 5-hydroxytryptamine receptor 2C (5-HT2C receptor) and prolactin receptor (PRLR), which were previously functionally correlated with qNSCs but not captured or profiled in RNAseq experiments. This study also identifies novel markers, including Kcnj13, a gene that encodes the potassium channel KiR7.1, that define the identity of these cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE311603 | GEO | 2026/06/09

REPOSITORIES: GEO

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