Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq analysis of 2C genes in mESC lines with overexpression of full length NELFA, MERVL-NELFA and IDR-deletion mutant (NELFA-IDR-Del).

ABSTRACT: Zygotic genome activation (ZGA) in early embryos is driven by extensive chromatin remodeling initiated by maternal factors, enabling the activation of cleavage-stage gene programs that underpin totipotency. In mouse embryonic stem cells (mESCs), a similar 2-cell-like (2C) state can be induced experimentally through the expression of specific maternal regulators such as NELFA. The RNA-seq dataset here was used to estimate the impact of full length NELFA, MERVL-NELFA and IDR-deletion mutant (NELFA-IDR-Del) on expression of 2C genes in mESCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE311875 | GEO | 2026/07/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-Seq analysis of 2C genes following MERVL-NELFA overexpression in wild type mESCs

Project description:Zygotic genome activation (ZGA) in early embryos is driven by extensive chromatin remodeling initiated by maternal factors, enabling the activation of cleavage-stage gene programs that underpin totipotency. In mouse embryonic stem cells (mESCs), a similar 2-cell-like (2C) state can be induced experimentally through the expression of specific maternal regulators such as NELFA. The RNA-seq dataset here was used to estimate the impact of NELFA-MERVL overexpression on of 2C characteristic genes in mESCs.

2026-07-27 | GSE311871 | GEO

RNA-Seq analysis of 2C genes in mESC lines with overexpression of full length NELFA, MERVL-NELFA and IDR-deletion mutant (NELFA-IDR-Del).

Project description:RNA-Seq analysis of 2C genes in mESC lines with overexpression of full length NELFA, MERVL-NELFA and IDR-deletion mutant (NELFA-IDR-Del).

| PRJNA1371879 | ENA

NELFA is a novel regulator of the 2C-like state

Project description:Mouse embryonic stem cells (mESCs) are not homogenous; a rare subpopulation of cells can sporadically transit into a early-embryonic-like state and exhibits a gene expression program resembling that of the 2-cell (2C) embryo. We identified the maternal factor NELFA as a novel driver of the 2C-like gene expression through multiple mechanisms. We further demonstrate that the entry of mESCs into the 2C-like state is accompanied by epigenetic and metabolic reprogramming, and that perturbation of the metabolic state via a small molecule can promote this cell fate reversion in a NELFA-dependent manner, obviating the need for any genetic manipulation. Our findings thus place NELFA as one of the earliest regulators of 2C gene expression, extending contemporary knowledge of how totipotency may be regulated.

2019-11-18 | GSE113671 | GEO

RNA-Seq analysis of 2C genes following Nelfa overexpression in H2AX WT or H2AX KO mESCs

Project description:Zygotic genome activation (ZGA) in early embryos is driven by extensive chromatin remodeling initiated by maternal factors, enabling the activation of cleavage-stage gene programs that underpin totipotency. In mouse embryonic stem cells (mESCs), a similar 2-cell-like (2C) state can be induced experimentally through the expression of specific maternal regulators such as NELFA. The RNA-seq dataset here was used to estimate the impact of NELFA overexpression on of 2C characteristic genes in context of H2AX KO in mESCs.

2026-07-27 | GSE311870 | GEO

RNA-Seq analysis of 2C genes in reporter mESC lines with or without pladienolide B treatment.

Project description:Zygotic genome activation (ZGA) in early embryos is driven by extensive chromatin remodeling initiated by maternal factors, enabling the activation of cleavage-stage gene programs that underpin totipotency. In mouse embryonic stem cells (mESCs), a similar 2-cell-like (2C) state can be induced experimentally through the expression of specific maternal regulators such as NELFA. The RNA-seq dataset here was used to estimate the impact of pladienolide B treatment in 3 different reporter mESC lines: 1 with NELFA reporter, 1 with NELFA-MERVL deletion and 1 with total NELFA KO.

2026-07-27 | GSE311873 | GEO

RNA-Seq analysis of 2C genes following MERVL-NELFA overexpression in wild type mESCs

Project description:RNA-Seq analysis of 2C genes following MERVL-NELFA overexpression in wild type mESCs

| PRJNA1371861 | ENA

NELFA is a novel regulator of the 2C-like state

Project description:NELFA is a novel regulator of the 2C-like state

| PRJNA453559 | ENA

RNA-Seq analysis of 2C genes following Nelfa overexpression in H2AX WT or H2AX KO mESCs

Project description:RNA-Seq analysis of 2C genes following Nelfa overexpression in H2AX WT or H2AX KO mESCs

| PRJNA1371862 | ENA

RNA-seq of Maternal Kdm1a mutant 2C embryos

Project description:2C embryos were generated from Kdm1a FL/Fl Zp3Cre females mated to wild type males for RNA-seq analysis using the Nugen Ovation RNA-seq system V2 3 replicates of maternal Kdm1a mutant 2C embryos were collected for RNA-seq , compared to wt 2C embryos and wt oocyte samples from Macfarlan TS et al, Nature 2012 (GEO acession GSE33923)

2015-03-10 | E-GEOD-66547 | biostudies-arrayexpress

MERVL/Zscan4 network activation results in transient genome-wide DNA demethylation of mESCs

Project description:Several previous studies have noted a rare sub-population of murine Embryonic Stem Cells (mESCs) which seem to express genes otherwise specific to the 2-cell stage of embryonic development. Here we sorted single cells from a MERVL/Zscan4 reporter line to investigate the continuum of expression as cells go from the standard state to this 2C-like state.

2016-09-30 | E-MTAB-5058 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data