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TRF-seq of DUS1L knockout and wild-type A375 human melanoma cells

ABSTRACT: Small RNA sequencing (tRF-seq) was performed on A375 human melanoma cells with CRISPR/Cas9-mediated knockout of DUS1L and wild-type control cells, with three biological replicates per group. Small RNA libraries were prepared using the NEBNext Small RNA Library Prep Set for Illumina and sequenced on an Illumina NextSeq 500 platform to generate single-end FASTQ files. Sequencing quality was assessed using FastQC, and 3' adaptor sequences were trimmed with cutadapt. Clean reads were aligned to the human tRNA genome using MINTmap to obtain read count-based expression profiles of tRNA-derived fragments (tRFs).

ORGANISM(S): Homo sapiens

PROVIDER: GSE312812 | GEO | 2025/12/12

REPOSITORIES: GEO

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