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Integrative omics analysis elucidates the role of ANKRD9 in modulating chicken primary myoblast IMP metabolism via the purine metabolic pathway

ABSTRACT: With the continuous improvement of human dietary structure, the demand for high-quality chicken meat is steadily increasing. It is therefore crucial for breeders to enhance meat quality while maintaining muscle yield in broilers. Inosine monophosphate (IMP) is a key compound influencing meat flavour and quality. In this study, we used the Chinese local breed Gushi chicken breed as the research model and observed dynamic expression changes of the ankyrin repeat domain 9 (ANKRD9) gene in breast muscle tissues. By integrating transcriptomic and metabolomic analyses, we systematically investigated the role of ANKRD9 in chicken primary myoblasts. Our findings indicate that ANKRD9 may affect cell adhesion and IMP metabolism through regulation of the purine metabolic pathway. Further cellular experiments confirmed that ANKRD9 inhibits IMP metabolism in myoblasts. These results highlight the critical role of ANKRD9 in regulating IMP, a key flavour-related metabolite, and provide a potential molecular target and theoretical foundation for the breeding of high-quality broilers.

ORGANISM(S): Gallus gallus

PROVIDER: GSE313870 | GEO | 2025/12/23

REPOSITORIES: GEO

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Project description:Improvement of feed efficiency would increase profitability of the poultry industries by decreasing the amount of feed required for production. Korat (KR) chicken is a new alternative meat-type chicken breed which its meat is recognized for its high protein, low fat and low purine content, whereas its low feed efficiency leads to high cost of production. Deeper understanding on how feed efficiency influences meat quality is poorly elucidated. To fulfill deeper understand molecular key that point the variation in feed efficiency and meat quality, the aim of this study was to investigate molecular pathways and genes involved in feed efficiency and meat quality in thigh of slow-growing KR chicken. A total of 75 males KR chicken were reared in individual cage until 10 weeks of age. Individual feed intake and body weight were collected weekly to calculate Feed Conversion Ratio (FCR) and Residual Feed Intake (RFI). Meat quality parameters were measured in thigh muscles such as ultimate pH (pHu), water-holding capacity (WHC), drip loss (DL), nucleotides content and several biomolecules (amide, …). Base on extreme values of FCR at 10 weeks of ages, 12 birds from the high FCR group (HFCR) and 9 birds from the low FCR group (LFCR) were selected for investigating their transcriptome using an 8×60K Agilent chicken microarray. In addition, a weighted gene coexpression network analysis was performed to detect the relationship between modules of co-expressed genes and feed efficiency, meat quality in thigh muscle. The result in this study indicated that selection on feed efficiency (FCR, RFI) would affect flavor precursor, lipid and protein content in thigh muscle. Based on WGCNA and functional enrichment analysis, results suggested that the key molecular pathways relate to FCR, RFI and meat quality (WHC, DL, IMP, AMP and inosine) in thigh muscle were the pathways of regulation of biological process, biological regulation and regulation of metabolic. Moreover, we revealed four genes there are assembly competence domain (ACD) gene, baculoviral IAP repeat containing 5 (BIRC5) gene, cytochrome c oxidase assembly factor 3 (COA3) gene and myosin light chain 9 (MYL9) gene that might be biomarker gene in feed efficiency and meat quality in thigh muscle. The hypothesis of the current study was alteration feed efficiency in slow-growing chicken will impact meat quality especially in term of texture and flavor.

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Integrative omics analysis elucidates the role of ANKRD9 in modulating chicken primary myoblast IMP metabolism via the purine metabolic pathway

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