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Temporal RNA-seq analysis of host transcriptional remodeling during lytic murine gammaherpesvirus 68 (MHV-68) infection

ABSTRACT: Bulk RNA sequencing was performed to characterize host transcriptional changes during lytic murine gammaherpesvirus 68 (MHV-68) infection. NIH 3T3 cells were mock- or MHV-68-infected (multiplicity of infection of 5) and harvested at 4, 8, 12, and 24 hours post-infection, representing immediate early through very late stages of lytic replication. Differential expression analysis revealed extensive and time-dependent remodeling of host gene expression, including immune, metabolic, chromatin-associated, and transcriptional pathways. This dataset provides a temporal framework for understanding host responses during productive lytic gammaherpesvirus infection.

ORGANISM(S): Mus musculus

PROVIDER: GSE314414 | GEO | 2026/05/13

REPOSITORIES: GEO

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