High-Resolution Spatial Transcriptomics of Irradiated Mouse Submandibular Gland Treated with Ep-FH

ABSTRACT: Our previous studies developed a novel scaffold by chemically conjugating laminin-1 peptides (A99 and YIGSR) and growth factors, FGF-7 and FGF-10, to fibrin hydrogels (Ep-FH). The resulting hydrogel, Ep-FH applied via a single intraglandular injection to irradiated mouse submandibular gland (SMG), enhanced epithelial tissue organization while promoted secretory function after 60 days of treatment. Together these results suggest a remarkable potential for clinical applications; however, before applying Ep-FH in a clinical setting, it is necessary to understand how the treatment affects different cell populations within SMG. Therefore, the goal of this study was to perform high-resolution spatial transcriptomics (Xenium 10 Genomics) to characterize how treatment with Ep-FH alters irradiated mouse SMG cell populations as well as relevant signaling mechanisms.

ORGANISM(S): Mus musculus

PROVIDER: GSE314531 | GEO | 2026/08/23

REPOSITORIES: GEO