Single-cell transcriptomic analysis reveals age-associated alterations in bone marrow niche-related cells
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ABSTRACT: Hematopoietic stem cells (HSCs) depend on the bone marrow (BM) niche for self-renewal and differentiation. Within this niche, CXCL12-abundant reticular (CAR) cells are key regulators of hematopoiesis and bone homeostasis. Single-cell RNA sequencing of adult and old mice revealed that old Adipo-CAR cells form a distinct cluster with reduced expression of ossification- and stem cell maintenance–related genes. RNA velocity and pseudotime analyses indicated impaired osteogenic differentiation, which was confirmed by in vitro assays showing diminished osteogenesis and increased adipogenesis. Structural and immunohistochemical analyses further demonstrated sinusoidal and endothelial abnormalities with age. These results suggest that age-related changes in Adipo-CAR cells and the sinusoidal niche underlie defective osteogenesis and hematopoietic decline.
ORGANISM(S): Mus musculus
PROVIDER: GSE315430 | GEO | 2026/02/03
REPOSITORIES: GEO
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