Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RXR binding pattern in human PMA-differentiated THP-1 cells upon ligand stimulation [ChIP-seq]

ABSTRACT: Retinoid X receptors (RXRs) act as obligate dimerisation partners for multiple nuclear receptors (NRs), each with distinct regulatory functions. In this study, human PMA-differentiated THP-1 (PMA-THP-1) cells were treated with single or combined ligands for RXR or six partners for 2 hours, and the genomic binding landscape of RXR was investigated using ChIP-seq. RXR genomic binding regions were also mapped following 1 hour of treatment with vehicle or 1,25-vitD using ChIP-seq. In addition, histone H3 lysine 27 acetylation (H3K27ac) was mapped following 6 hours of treatment with vehicle or 1,25-vitD using ChIP-seq. In parallel, RNA-seq was performed on PMA-THP-1 cells following 6 hours of treatment with vehicle, 1,25-vitD or combined ligands to identify and compare the regulated gene sets.

ORGANISM(S): Homo sapiens

PROVIDER: GSE315571 | GEO | 2026/05/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RXR binding pattern in human PMA-differentiated THP-1 cells upon ligand stimulation [RNA-seq]

Project description:Retinoid X receptors (RXRs) act as obligate dimerisation partners for multiple nuclear receptors (NRs), each with distinct regulatory functions. In this study, human PMA-differentiated THP-1 (PMA-THP-1) cells were treated with single or combined ligands for RXR or six partners for 2 hours, and the genomic binding landscape of RXR was investigated using ChIP-seq. RXR genomic binding regions were also mapped following 1 hour of treatment with vehicle or 1,25-vitD using ChIP-seq. In addition, histone H3 lysine 27 acetylation (H3K27ac) was mapped following 6 hours of treatment with vehicle or 1,25-vitD using ChIP-seq. In parallel, RNA-seq was performed on PMA-THP-1 cells following 6 hours of treatment with vehicle, 1,25-vitD or combined ligands to identify and compare the regulated gene sets.

2026-05-14 | GSE315572 | GEO

Transcriptome profiling of genes regulated by RXR and its partners in monocyte-derived dendritic cells

Project description:CD14+ human monocytes differentiating into DCs in the presence of IL4 and GM-CSF were treated with agonists for RXR and its partners or vehicle 18 hours after plating (experiment with RXR and permissive partners, donor 1-3) or 14 hours after plating (experiment with nonpermissive partners, donor 4-6). Cells were harvested 12 hours thereafter. Experiments were performed in biological triplicates representing samples from three different donors. In this study all probable RXR-signaling pathways induced by agonists for RXR, LXRs, PPARs, RAR and VDR were identified in differentiating human monocyte-derived dendritic cells. In the experiments, differentiating dendritic cells were treated for 12 hours with one of the following compounds (ligands): vehicle (DMS:EtOH 1:1) LG268 (RXR agonist) 9-cis retinoic acid (9cisRA, agonist of RAR and RXR) GW3965 (LXRalpha/beta panagonist) rosiglitazone (RSG, PPARgamma agonist) GW1516 (PPARdelta agonist) AM580 (RARalpha agonist) 1,25-dihydroxyvitamin D3 (VDR agonist)

2010-09-01 | E-GEOD-23073 | biostudies-arrayexpress

Genomic regions occupied by both RARα and VDR are involved in the convergence and cooperation of retinoid and vitamin D signaling pathways.

Project description:In this study, the retinoid and vitamin D pathways were investigated in PMA-differentiated THP-1 cells treated with or without the RARa agonist, AM580, and/or the VDR agonist, 1,25-dihydroxyvitamin D3 (1,25-vitD or calcitriol), using global approaches. Binding regions of RARa and VDR were determined in PMA-differentiated THP-1 cells using chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq). The gene sets regulated by AM580 and/or 1,25-vitD were identified by RNA-sequencing (RNA-seq). The activity of the regulatory regions was determined by MED1 (a subunit of the Mediator complex) ChIP-seq and chromatin openness was measured using assay for transposase-accessible chromatin with sequencing (ATAC-seq).

2024-12-31 | GSE246309 | GEO

Genomic regions occupied by both RARα and VDR are involved in the convergence and cooperation of retinoid and vitamin D signaling pathways.

2024-12-31 | GSE246308 | GEO

Genomic regions occupied by both RARα and VDR are involved in the convergence and cooperation of retinoid and vitamin D signaling pathways.

2024-12-31 | GSE246307 | GEO

Transcriptome profiling of genes regulated by RXR and its partners in monocyte-derived dendritic cells

2010-09-01 | GSE23073 | GEO

Human mRNA profile of Mycobacterium tuberculosis H37Rv infected and hsa-miR-643 mimic transfected THP-1 differentiated macrophages cells.

Project description:The analysis of differentially regulated mRNAs candidates in THP-1 cells infected with H37Rv was conducted, comparing them to both uninfected THP-1 cells and transfected reference samples. THP-1 cells are monocytes that differentiate into macrophages after being treated with PMA for 48 hours. Total RNA was isolated from the infected and transfected THP-1 cells at 24 hours post-infection.

2025-04-17 | GSE275580 | GEO

Mitochondrial Transplantation with Vitamin D Therapy Mitigates Paraspinal Muscle Atrophy and Neuropathic Pain Following Spinal Surgery

Project description:Chronic pain after spinal surgery (CPSS) is frequently accompanied by paraspinal muscle atrophy (PMA). This study aimed to explore the potential of mitochondrial transplantation and/or vitamin D (VitD) therapy to counteract PMA- and pain-associated mitochondrial dysfunction. Plasma-derived mitochondria (pMT) were collected from human blood samples. The structure, size, and purity of the pMT were verified, and their mitochondrial respiratory enzyme activity was analyzed. A 2-week rat model of CPSS was created at the fifth left lumbar vertebra through denervation and laminectomy. The rats were assigned to 5 groups, namely, pMT/VitD, pMT, VitD, surgery, and control and administered intramuscular pMT ± VitD injections. The effects elicited in each group were investigated after 2 weeks via muscle morphology measurement and comprehensive tissue analysis. Combined pMT and VitD treatments showed potential in alleviating surgery-induced muscle atrophy and modulating pain responses. Thus, pMT and/or VitD treatment restored muscle atrophy by modulating proteostasis, suppressing apoptosis, and increasing PGC1α levels.

2025-09-24 | GSE267096 | GEO

Effects of PMA on exon usage of HL60, THP-1, and U937 cells

Project description:Myeloid leukemia cell lines HL60, THP-1, and U937 undergo macrophage-like differentiation after treatment with phorbol ester. To explore genes whose exon usage was altered during macrophage differentiation, we compared exome of PMA-treated (differentated) and vehicle-treated (undifferentiated) myeloid cell lines. HL60, THP-1, and U937 cells were treated with either phorbol 12-myristate 13-acetate (PMA,30nM) or its vehicle (DMSO) for 3 days, and subjected to exome analysis using Affymetrix human exon 1.0ST arrays.

2013-09-25 | E-GEOD-20567 | biostudies-arrayexpress

Pioglitazone competes with EGR1 to suppress PKD1 in Angiotensin II-treated macrophages

Project description:THP-1 cells were differentiated to macrophages with the phorbol 12-myristate 13-acetate (PMA) for a total time of 28 hours. To understand the effects of Angiotensin II (AngII) and of AngII in the presence of the thiazolidinedione pioglitazone (Pio), after 4 hours of PMA-induced differentiation of THP-1 cells (when cells became adherent), the PMA-containing medium was supplemented with AngII (1mg/ml), AngII (1 mg/ml) with Pio (1 uM), or no supplement for another 24 hours. At this time point the cells were harvested, RNA was extracted and used for a microarray-based gene profiling analysis of THP-1 cells, under the three conditions: PMA, AngII and AngII with Pio.

2014-06-01 | E-MTAB-2320 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data