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Single-cell sequencing and spatial transcriptomics revealed that the coacervate–cell–drug system effectively protects articular cartilage in osteoarthritic rats from wear and degenerative deterioration.

ABSTRACT: We employed DNBSEQ technology to perform single-cell RNA sequencing of rat macrophages, as well as single-cell and spatial transcriptomic analyses of rat knee joint tissues. Single-cell profiling of macrophages demonstrated that the coacervate–cell–drug system effectively reversed the pro-inflammatory phenotype by driving the transition of M1 macrophages toward a reparative M2c phenotype. Single-cell analysis of joint tissues further revealed strong interactions between exogenous GFP-labeled stem cells delivered by the coacervate–cell–drug system and endogenous joint-resident cells. Spatial transcriptomic analysis demonstrated that the coacervate–cell–drug system effectively promotes extracellular matrix repair in osteoarthritic cartilage.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE316007 | GEO | 2026/06/27

REPOSITORIES: GEO

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Single-cell sequencing and spatial transcriptomics revealed that the coacervate–cell–drug system effectively protects articular cartilage in osteoarthritic rats from wear and degenerative deterioration.

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