Identification progenitor Keratinocyte Subpopulations in HaCaT via high throughput sequencing

ABSTRACT: Epidermal progenitor cells rely on tightly regulated transcriptional and epigenetic programs to preserve cellular plasticity and prevent premature differentiation; however, these regulatory mechanisms remain incompletely characterized. In this study, we investigated the transcriptomic architecture of progenitor keratinocyte subpopulations isolated from HaCaT cells using fluorescence-activated cell sorting and bulk RNA sequencing. Differential expression analysis revealed distinct protein-coding and long non-coding RNA (lncRNA) profiles between subpopulations enriched for progenitor-associated markers and more differentiated cells. Enrichment analyses providing a transcriptomic framework for exploring epigenetic regulation of keratinocyte progenitor identity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE317695 | GEO | 2026/01/31

REPOSITORIES: GEO