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LRMDA suppresses colonic inflammation, colorectal cancer and bacterial infection [Colitis_RNA-seq]

ABSTRACT: The leucine-rich melanocyte differentiation-associated protein (LRMDA), a recently characterized intracellular trafficking regulator, plays a critical yet unexplored role in intestinal homeostasis 1. Here, we show that LRMDA expression is downregulated in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and in colorectal tumor tissues. Similarly, we demonstrate that LRMDA deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis, promotes tumorigenesis in azoxymethane (AOM)/DSS and AOM/Vil-Cre;Trp53fl/fl (VP) models and aggravates enteric pathogen infection. Mechanistically, LRMDA may positively regulate the normal exocytosis of mucins from intestinal goblet cells into the intestinal lumen via the dynein-dynactin-microtubule pathway by interacting with the ICD domain of DCTN1. These findings identify LRMDA as a novel regulator of intestinal mucosal barrier function.

ORGANISM(S): Mus musculus

PROVIDER: GSE317857 | GEO | 2026/05/01

REPOSITORIES: GEO

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LRMDA suppresses colonic inflammation, colorectal cancer and bacterial infection [CRC_RNA-seq]

Project description:The leucine-rich melanocyte differentiation-associated protein (LRMDA), a recently characterized intracellular trafficking regulator, plays a critical yet unexplored role in intestinal homeostasis 1. Here, we show that LRMDA expression is downregulated in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and in colorectal tumor tissues. Similarly, we demonstrate that LRMDA deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis, promotes tumorigenesis in azoxymethane (AOM)/DSS and AOM/Vil-Cre;Trp53fl/fl (VP) models and aggravates enteric pathogen infection. Mechanistically, LRMDA may positively regulate the normal exocytosis of mucins from intestinal goblet cells into the intestinal lumen via the dynein-dynactin-microtubule pathway by interacting with the ICD domain of DCTN1. These findings identify LRMDA as a novel regulator of intestinal mucosal barrier function.

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