RNA-seq analysis of B16F10 cells following p300/CBO knockout

ABSTRACT: The p300 and CBP (CREB-binding protein) paralogs are essential histone acetyltransferases (HATs) that serve as global transcriptional coactivators. In many cancers, including melanoma, these proteins redundantly regulate key oncogenic and lineage-specific gene programs. To investigate their combined role in melanoma, we generated p300/CBP sinlge and double-knockout (dKO) B16F10 murine melanoma cells using CRISPR/Cas9. We then performed bulk RNA-sequencing to identify genes and biological pathways that are critically dependent on these coactivators. Our results define the shared and unique transcriptional targets of p300/CBP in the B16F10 model and provide a resource for understanding how their loss impacts melanoma cell fitness, invasive potential, and stress responses.

ORGANISM(S): Mus musculus

PROVIDER: GSE318796 | GEO | 2026/04/29

REPOSITORIES: GEO