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Single-nucleus transcriptomics of CLRN1 knockout rabbit retina reveals Müller glia-driven mechanisms of retinal degeneration

ABSTRACT: Single-nucleus RNA sequencing of wild-type and CLRN1 knockout rabbit retina at 10 months of age (pre-symptomatic timepoint). Three biological replicates per genotype were pooled and processed using 10x Genomics Chromium Single Cell 3' v3.1 platform. SNP-based demultiplexing with Vireo was used to assign nuclei to individual animals. Analysis of 6,542 high-quality nuclei across 7 retinal cell types revealed that CLRN1 is exclusively expressed in Müller glia and identified a primary adherens junction defect (CTNNA2 downregulation) leading to secondary photoreceptor degeneration while inner retinal neurons mount robust compensatory responses.

ORGANISM(S): Oryctolagus cuniculus

PROVIDER: GSE320260 | GEO | 2026/07/08

REPOSITORIES: GEO

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