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Short-Duration HIPEC-Mimetic Exposure to Mithramycin A Induces Structured Transcriptional Reprogramming in HT-29 Colorectal Cancer Cells

ABSTRACT: This study investigates the transcriptional consequences of short-duration HIPEC-mimetic exposure to Mithramycin A (MA) in HT-29 colorectal cancer cells. Cells were exposed for 90 minutes to MA (750 nM) or Mitomycin C (4.48 µM) at 37 °C followed by drug washout. RNA sequencing was performed to determine whether transient exposure induces durable transcriptional reprogramming. Differential expression and pathway analyses identified coordinated enrichment of cell-cycle checkpoint programs and modulation of chromatin regulatory networks. One biological replicate (MA2) was excluded due to high duplication rates and low mapping efficiency. Analyses excluding this sample are presented.

ORGANISM(S): Homo sapiens

PROVIDER: GSE320366 | GEO | 2026/05/15

REPOSITORIES: GEO

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