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Effect of BHLHE40 silencing using short-hair pin RNA on CD34+ cells isolated from human cord blood, during TGF-B1 depedent Langerhans cell differentiation

ABSTRACT: Langerhans cells (LCs) are resident antigen presenting cells of the epidermis whose differentiation, immature state and tissue residency critically depend on transforming growth factor–β1 (TGF-β1) signaling. Although several downstream transcriptional regulators of TGF-β1 have been identified, how LC epithelial identity and homeostasis are transcriptionally stabilized remains incompletely understood.Human CD34⁺ hematopoietic progenitors were differentiated into LCs or monocyte-derived cells in vitro using cytokine-defined conditions, combined with lentiviral knockdown or ectopic expression of BHLHE40. Bulk-RNA seq of shBHLHE40 cells reveals blunted induction of TGF-β1 responsive genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE324988 | GEO | 2026/05/01

REPOSITORIES: GEO

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