Influence of lateral compression on epithelium and role of lamin A in this process

ABSTRACT: Intercellular compressive forces during cellular packing and maturation of epithelium are guided by cellular mechanotransduction. In this study, the goal was to identify how lateral compression alters gene expression in the epithelium, and given that nuclear lamina -mediated nuclear mechanics contribute in mechanotransduction, we sought to decipher the role of A-type lamins in this process. We used Madine Darby canine kidney type II (MDCKII) cells as wild-type (WT) cells and established a derivative pre-lamin A knockout (referred here as LMNA KO) cell line. Cells were grown for 3-4 days until an intact monolayer was formed (but which had not reached maturity). We applied our in house developed Brick Strex cell stretching deviceand cultured cells in this device on a pre-stretched and pre-strained PDMS with 25 % tensile strain, inducing a 20 % lateral compressive strain upon gradual release of the pre-strain. After a 2 h recovery, total cell lysates were made and mRNA was extracted for mRNA-seq.

ORGANISM(S): Canis lupus familiaris

PROVIDER: GSE325464 | GEO | 2026/03/24

REPOSITORIES: GEO