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Stem cell-derived exosomes inhibit the inflammatory response of microglia in neuropathic pain through m6A modification mechanisms

ABSTRACT: Activated microglia-induced neuroinflammation is a critical pathological basis for the exacerbation of neuropathic pain. In our in vitro experiments, we found that exosomes derived from human umbilical cord mesenchymal stem cells exhibit a potent inhibitory effect on microglial inflammation, significantly downregulating the expression of lipopolysaccharide (LPS)-induced inflammatory factors such as interleukin-6 and interleukin-1, and suppressing microglial activation. However, the underlying molecular mechanism requires further investigation. m6A modification is considered one of the key molecular mechanisms regulating neuropathic pain, but whether stem cell-derived exosomes inhibit microglial inflammation through modulating m6A methylation remains to be further elucidated. Therefore, this study aims to investigate changes in m6A modification following exosome treatment, identify key m6A-modified genes and the regulatory enzymes involved, and use them as research targets to explore the molecular mechanism by which stem cell-derived exosomes alleviate neuropathic pain.

ORGANISM(S): Mus musculus

PROVIDER: GSE326825 | GEO | 2026/04/07

REPOSITORIES: GEO

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