Modelling B cell development with pluripotent stem cells

ABSTRACT: The ability to generate functional B cells from human pluripotent stem cells (hPSCs) would open new opportunities to develop novel B cell-based therapies to treat a range of human diseases and disorders. Towards this goal, we established a MS-5 stromal based protocol that promotes the efficient development of B lineage cells from definitive hematopoietic progenitors generated from different hPSC lines. Flow cytometric and multi-omic scRNA-seq analyses revealed that B cell development from hPSCs transitions through the well-established pro- B, pre-B and naïve B cell stages, accurately recapitulating B lymphopoiesis in the human adult bone marrow.

ORGANISM(S): Homo sapiens

PROVIDER: GSE327194 | GEO | 2026/08/21

REPOSITORIES: GEO