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Transcriptomic analysis of HEK-293 cells subjected to prolonged mild acidification (pH 6.6) and treatment with an SR protein kinase inhibitor (SRPIN340)

ABSTRACT: Acidosis, the hallmark of aggressive cancer and chronic inflammation, is associated with altered transcription and alternative splicing. These alterations may be due to the repressed release of transcription and splicing factors from splicing speckles, which are scaffolded by phosphorylated SR-rich proteins. We aimed to partially verify this hypothesis and clarify whether changes in SR solubility caused by acidification and dephosphorylation have any common effects on transcription and alternative splicing. For this, we modelled prolonged (2 d) non-damaging acidosis in non-synchronized HEK-293 cells and compared the transcriptomic changes to those induced by a subtoxic concentration of a specific SR protein kinase inhibitor SRPIN340.

ORGANISM(S): Homo sapiens

PROVIDER: GSE328432 | GEO | 2026/04/22

REPOSITORIES: GEO

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