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Single-cell RNA analysis of allele-specific MHC expression in macaque PBMC after T cell activation or treatment with pro-inflammatory or anti-inflammatory cytokines

ABSTRACT: We have employed a single-cell sequencing approach using 10X Genomics 5'scRNAseq to study expression of the Major Histocompatibility Complex (MHC) in peripheral blood mononuclear cells (PBMC) from Mauritian-origin cynomolgus and Indian-origin rhesus macaques. The MHC region has undergone a complex series of duplications in macaques relative to humans. Steady-state RNA levels of macaque MHC class I and class II transcripts vary over several orders of magnitude in whole blood and bulk PBMC. In this study, we compared transcript levels in untreated control PBMC with transcript levels after T cell activation, treatment with pro-inflammatory cytokines, or treatment with anti-inflammatory cytokines. Transcript levels for several minor MHC alleles were preferentially induced in monocytes and dendritic cells after treatment with pro-inflammatory cytokines or T cell activation.

ORGANISM(S): Macaca mulatta Macaca fascicularis

PROVIDER: GSE329692 | GEO | 2026/05/04

REPOSITORIES: GEO

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