Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spatial Transcriptomics of MHC Expression in Mauritian Cynomolgus Macaque Tissues

ABSTRACT: A spatial transcriptomics method was developed with the 10X Genomics Visium HD platform and a custom probe panel to study expression of Major Histocompatibility Complex (MHC) genes in tissues from Mauritian-origin cynomolgus macaques. Previous scRNA sequencing studies with these macaque demonstrated a wide range of allele-specific MHC transcript levels in multiple peripheral blood mononuclear cell subsets. The restricted genetic diversity of the Mauritian macaque population made it possible to design a manageable number of custom MHC probes for this pilot study . Tissues examined included kidney, liver, lung and heart that of particular interest for transplant research. The Visium Human Transcriptome Kit v2 performed well with at least 40 different kidney cell types identified based on cell clustering with gene expression definitions in a human kidney cell atlas. In addition, higher overall transcript levels were observed for several major MHC class I alleles compared to minor alleles that tend to be expressed at low levels in peripheral blood mononuclear cells.

ORGANISM(S): Macaca fascicularis

PROVIDER: GSE336633 | GEO | 2026/06/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell RNA analysis of allele-specific MHC expression in peripheral blood mononuclear cells of Mauritian-origin cynomologus and Indian-origin rhesus macaques

Project description:We have employed a single-cell sequencing approach using 10X Genomics 5'scRNAseq to study expression of the Major Histocompatibility Complex (MHC) in peripheral blood mononuclear cells (PBMC) from Mauritian-origin cynomolgus and Indian-origin rhesus macaques. The MHC region has undergone a complex series of duplications in macaques relative to humans. Steady-state RNA levels of macaque MHC class I and class II transcripts vary over several orders of magnitude in whole blood and bulk PBMC. This study represents the first 5'scRNAseq analysis to quantify allele-specific levels of MHC transcripts in various PMBC subsets of macaques and provides insights into how they may shape immune responses.

2026-03-16 | GSE324697 | GEO

Single-cell RNA analysis of allele-specific MHC expression in macaque PBMC after T cell activation or treatment with pro-inflammatory or anti-inflammatory cytokines

Project description:We have employed a single-cell sequencing approach using 10X Genomics 5'scRNAseq to study expression of the Major Histocompatibility Complex (MHC) in peripheral blood mononuclear cells (PBMC) from Mauritian-origin cynomolgus and Indian-origin rhesus macaques. The MHC region has undergone a complex series of duplications in macaques relative to humans. Steady-state RNA levels of macaque MHC class I and class II transcripts vary over several orders of magnitude in whole blood and bulk PBMC. In this study, we compared transcript levels in untreated control PBMC with transcript levels after T cell activation, treatment with pro-inflammatory cytokines, or treatment with anti-inflammatory cytokines. Transcript levels for several minor MHC alleles were preferentially induced in monocytes and dendritic cells after treatment with pro-inflammatory cytokines or T cell activation.

2026-05-04 | GSE329692 | GEO

Spatial Transcriptomics of MHC Expression in Mauritian Cynomolgus Macaque Tissues

Project description:Spatial Transcriptomics of MHC Expression in Mauritian Cynomolgus Macaque Tissues

| PRJNA1482125 | ENA

Cytomegalovirus Vaccine-induced Unconventional T cell Priming and Protection against SIV Replication is Conserved Between Primate Species

Project description:Bulk RNA Sequencing of Mauritian cynomolgus macaque (MCM) whole blood pre- and post-vaccination with CyCMV/SIV

2022-12-21 | GSE205080 | GEO

CD8+ cells and small viral reservoirs facilitate post-ART SIV control in MHC-M3+ Mauritian cynomolgus macaques

Project description:Sustainable HIV remission after antiretroviral therapy (ART) withdrawal, or post-treatment control (PTC), remains a top priority in the HIV field. We observed surprising PTC in an MHC-haplomatched cohort of SIVmac239+ Mauritian cynomolgus macaques (MCMs) initiated on ART at two weeks post-infection (wpi). For six months after ART withdrawal, we observed undetectable or transient viremia in seven of eight MCMs. In vivo CD8α depletion induced rebound in all animals, indicating the PTC was mediated by CD8α+ cells. We found that MCMs had a smaller acute viral reservoir than a cohort of identically infected rhesus macaques, a population that rarely develops PTC. The mechanisms by which unusually small viral reservoirs and CD8α+ cell-mediated virus suppression enable PTC can be investigated using this model of PTC in MHC-haplomatched MCMs. If the immunological mechanisms that can engender PTC are defined, this model could designate therapeutic targets for HIV remission strategies in humans.

2023-08-07 | GSE225770 | GEO

Immunomodulatory potential of in vivo Natural Killer T (NKT) activation by NKTT320 in Mauritian-origin cynomolgus macaques

Project description:Invariant natural killer T-lymphocytes (iNKT) are unique immunomodulatory innate T-cells with an invariant TCR recognizing glycolipids presented on MHC class-I-like CD1d molecules. Activated iNKT rapidly secrete pro-and anti-inflammatory cytokines, potentiate immunity, and modulate inflammation. Here, we report the effects of in vivo iNKT activation in Mauritian-origin cynomolgus macaques by a humanized monoclonal antibody, NKTT320, that binds to the invariant region of the iNKT TCR. NKTT320 led to rapid iNKT activation, increased polyfunctionality, and elevation of multiple plasma analytes within 24 hours of administration. Flow cytometry and RNA-Seq confirmed downstream activation of multiple immune subsets, enrichment of JAK/STAT and PI3K/AKT pathway genes, and upregulation of inflammation-modulating genes. NKTT320 also increased iNKT frequency in adipose tissue without iNKT anergy. Our data indicate that NKTT320 has a sustained effect on in vivo iNKT activation, potentiation of innate and adaptive immunity, and resolution of inflammation, which supports its future use as an immunotherapeutic.

2022-01-25 | GSE194235 | GEO

Single-cell RNA analysis of allele-specific MHC expression in peripheral blood mononuclear cells of Mauritian-origin cynomologus and Indian-origin rhesus macaques

Project description:Single-cell RNA analysis of allele-specific MHC expression in peripheral blood mononuclear cells of Mauritian-origin cynomologus and Indian-origin rhesus macaques

| PRJNA1436580 | ENA

Mafa-MHC

Project description:Mafa-MHC alleles

| PRJEB43388 | ENA

CD8+ cells and small viral reservoirs facilitate post-ART SIV control in MHC-M3+ Mauritian cynomolgus macaques

Project description:CD8+ cells and small viral reservoirs facilitate post-ART SIV control in MHC-M3+ Mauritian cynomolgus macaques

| PRJNA937379 | ENA

Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

Project description:HLA-DRB1 alleles have been associated with several autoimmune diseases. In anti-citrullinated protein antibody positive rheumatoid arthritis (ACPA-positive RA), HLA-DRB1 shared epitope (SE) alleles are the major genetic risk factors. In order to investigate whether expression of different alleles of major histocompatibility complex (MHC) Class II genes influence functions of immune cells, we investigated transcriptomic profiles of a variety of immune cells from healthy individuals carrying different HLA-DRB1 alleles. Sequencing libraries from peripheral blood mononuclear cells, CD4+ T cells, CD8+ T cells, and CD14+ monocytes of 32 genetically pre-selected healthy female individuals were generated, sequenced and reads were aligned to the standard reference. For the MHC region, reads were mapped to available MHC reference haplotypes and AltHapAlignR was used to estimate gene expression. Using this method, HLA-DRB and HLA-DQ were found to be differentially expressed in different immune cells of healthy individuals as well as in whole blood samples of RA patients carrying HLA-DRB1 SE-positive versus SE-negative alleles. In contrast, no genes outside the MHC region were differentially expressed between individuals carrying HLA-DRB1 SE-positive and SE-negative alleles. Existing methods for HLA-DR allele-specific protein expression were evaluated but were not mature enough to provide appropriate complementary information at the protein level. Altogether, our findings suggest that immune effects associated with different allelic forms of HLA-DR and HLA-DQ may be associated not only with differences in the structure of these proteins, but also with differences in their expression levels.

2020-12-22 | GSE163605 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data