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Expression data from OT-I CD8 T cells that are either naïve or isolated on day 5 following immunization with DC-OVA or with DC-OVA + LCMV

ABSTRACT: Inflamatory cytokines have a number of effects on the differentiation and function of CD8 T cells. To separate the effects of TCR/co-stimulation from the effects of inflammatory cytokines, we took an immunization approach. We transferred 1 x 10^4 OT-I CD8 T cells to naive C57BL/6 mice on day -1. On day 0 mice were immunized with either 5 x 10^5 OVA peptide loaded, LPS-matured dendritic cells (DC-OVA) injected ip or 5 x 10^5 DC-OVA and 2 x 10^5 pfu of LCMV injected ip. On day 5 following the indicated treatment, OT-I CD8 T cells were enriched via AutoMACS and sorted via flow cytometry prior to RNA extraction from 1 x 10^6 cells using an RNeasy kit (Qiagen). Naive OT-I CD8 T cells were enriched and sorted for RNA extraction to serve as a control. Each group had 3 biological replicates.

ORGANISM(S): Mus musculus

PROVIDER: GSE331111 | GEO | 2026/06/01

REPOSITORIES: GEO

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Expression data from OT-I CD8 T cells activated with DC in the presence or absence of CpG-Induced Inflammation

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2013-12-10 | E-GEOD-53200 | biostudies-arrayexpress

Expression data from OT-I CD8 T cells activated with DC in the presence or absence of CpG-Induced Inflammation

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