Distinct media requirements for dopaminergic neuron reprogramming from human glia and fibroblasts

ABSTRACT: Bulk RNA sequencing was performed on human glial progenitor cells (hGPCs) derived from the hESC line RC17 and reprogrammed toward a dopaminergic neuron fate using Ascl1, Lmx1a, Nurr1 and shREST (ALNRi). Cells were cultured under two media conditions; standard NDiff medium or a B27/Neurobasal-based medium without small molecules (new media) and harvested at day 10 and day 21 post-transduction (three technical replicates per condition). The dataset was generated to identify transcriptional differences underlying the improved dopaminergic reprogramming efficiency observed with the new media. Gene-level counts were quantified using Salmon (alignment-based mode, GRCh38/hg38) and merged across samples using tximport.

ORGANISM(S): Homo sapiens

PROVIDER: GSE333803 | GEO | 2026/08/21

REPOSITORIES: GEO