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Direct RNA sequencing of in vitro transcribed eGFP mRNA modified with 5-methoxyuracil

ABSTRACT: The aim of this study was to understand potential biases in the incorporation of modified nucleotides, specifically 5-methoxyuracil, in in vitro transcription reactions by different T7 polymerases through Nanopore Direct RNA Sequencing. Incorporation by a range of T7 polymerases using different DNA templates was sequenced using RNA004 flow cell chemistry. Analysis was performed using basecall error-based modification detection (EpiNano).

ORGANISM(S): synthetic construct

PROVIDER: GSE335071 | GEO | 2026/08/20

REPOSITORIES: GEO

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Direct RNA sequencing of in vitro transcribed mRNA modified with 5-methoxyuracil

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