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A global regulatory atlas of Streptomyces reveals conserved and diversified transcriptional networks across actinomycetes

ABSTRACT: Here, we apply DNA affinity purification sequencing (DAP-seq) to 789 31 predicted TFs of Streptomyces coelicolor, generating genome-wide binding maps for 393 32 regulators and expanding the experimentally supported regulome from ~8% to ~50%. 33 Integration with ChIP-seq reveals pleiotropic regulators and hierarchical network architecture 34 linking primary metabolism, development and biosynthetic gene clusters (BGCs). This GEO data set include 11 ChIP-seq results discussed in our manuscript

ORGANISM(S): Streptomyces coelicolor A3(2)

PROVIDER: GSE337352 | GEO | 2026/07/02

REPOSITORIES: GEO

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Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor

Project description:A complex programme of regulation governs gene expression during development of the morphologically and biochemically complex eubacterial genus Streptomyces. Earlier work has suggested a model in which 'higher level' pleiotropic regulators activate 'pathway-specific' regulators located within chromosomal gene clusters encoding biosynthesis of individual antibiotics. We used mutational analysis and adventitious overexpression of key Streptomyces coelicolor regulators to investigate functional interactions among them. We report here that cluster-situated regulators (CSRs) thought to be pathway-specific can also control other antibiotic biosynthetic gene clusters, and thus have pleiotropic actions. Surprisingly, we also find that CSRs exhibit growth-phase-dependent control over afsR2/afsS, a 'higher level' pleiotropic regulatory locus not located within any of the chromosomal gene clusters it targets, and further demonstrate that cross-regulation by CSRs is modulated globally and differentially during the S. coelicolor growth cycle by the RNaseIII homologue AbsB. Our results, which reveal a network of functional interactions among regulators that govern production of antibiotics and other secondary metabolites in S. coelicolor, suggest that revision of the currently prevalent view of higher-level versus pathway-specific regulation of secondary metabolism in Streptomyces species is warranted. Groups of assays that are related as part of a time series. Keywords: time_series_design

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Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor

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