Methylation profiling

Dataset Information

0

Protocol for low-input whole-genome bisulfite sequencing in plants


ABSTRACT: DNA methylation profiling of rare plant samples is limited by the scarcity of input DNA. This protocol uses Arabidopsis leaf to describe tissue cryo-milling, proteinase K lysis, direct bisulfite conversion, post-bisulfite adapter tagging library construction with random priming and adapter ligation, and MethylStar-based alignment and methylation calling. DNA inputs as low as 50 pg generate methylomes comparable to conventional 50-ng WGBS.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE338396 | GEO | 2026/07/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-04-14 | GSE112875 | GEO
2025-09-27 | GSE294197 | GEO
2025-01-17 | GSE286342 | GEO
2022-07-15 | GSE201829 | GEO
2020-08-20 | GSE71700 | GEO
2015-04-21 | E-ERAD-384 | biostudies-arrayexpress
2015-02-01 | E-GEOD-55850 | biostudies-arrayexpress
2018-02-15 | GSE88716 | GEO
2015-01-31 | GSE59330 | GEO
2014-12-31 | GSE63523 | GEO