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Single-cell transcriptomic profiling of Toxoplasma gondii eIF5A K49 mutants during alkaline stress-induced differentiation

ABSTRACT: This study investigates how the acetylation status of lysine 49 (K49) in eukaryotic translation initiation factor 5A (eIF5A) influences transcriptional heterogeneity and developmental-state transitions in Toxoplasma gondii. Using the ME49 strain as the genetic background, we performed 10x Genomics single-cell RNA sequencing on wild-type parasites, an eIF5A-K49Q acetylation-mimic mutant, and an eIF5A-K49R non-acetylatable charge-conservative mutant. Each parasite line was cultured under standard conditions or exposed to alkaline medium (pH 8.2 for 4 days) to induce tachyzoite-to-bradyzoite differentiation in vitro. For each genotype-by-condition group, three independently cultured biological replicates were pooled before single-cell capture to generate one scRNA-seq library. Six pooled libraries were generated in total. This dataset enables characterization of parasite cell-state heterogeneity and transcriptional programs associated with eIF5A K49 status during stress-induced differentiation.

ORGANISM(S): Toxoplasma gondii

PROVIDER: GSE346252 | GEO | 2026/09/05

REPOSITORIES: GEO

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