Functional genetic screening identifies ITGA3 as a regulator of pancreatic ductal adenocarcinoma growth and organoid architecture

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers, with limited therapeutic options. In this study, we performed a targeted CRISPR screen and identified integrin a3 (ITGA3) as a dependency. ITGA3 knockdown resulted in reduced organoid cell viability, loss of luminal structure, and decreased invasion in collagen I. Transcriptomic analysis revealed that ITGA3 knockdown resulted in upregulation of cilia-related pathways, a finding not previously reported. ITGA3 knockdown also reduced tumor growth in vivo. This study expands on the role of ITGA3 in promoting aggressive cancer phenotypes and provides a novel link to cilia-related transcriptional programs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE349252 | GEO | 2026/10/01

REPOSITORIES: GEO