Transcriptomics,Genomics

Dataset Information

48

Derivation of small molecule Human Neural Precursor Cells (smNPCs) from human pluripotent stem cell lines


ABSTRACT: We have developed a method for derivation of smNPCs from human embryonic (hESC) and induced pluripotent stem cells (hIPSC), that are only dependent on small molecules stimulating WNT and SHH signalling for their propagation. These smNPC cultures were enzymatically split every five to six days. Overall design: Human pluripotent stem cells were differentiated into the neural lineage using double SMAD inhibition, together with stimulation of WNT and SHH pathways. This led to expansion of neural epithelium, which was further cultured for 20 passages.

INSTRUMENT(S): Illumina HumanHT-12 V4.0 expression beadchip

SUBMITTER: Peter Reinhardt  

PROVIDER: GSE40556 | GEO | 2013-03-22

SECONDARY ACCESSION(S): PRJNA174291

REPOSITORIES: GEO

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Publications


Phenotypic drug discovery requires billions of cells for high-throughput screening (HTS) campaigns. Because up to several million different small molecules will be tested in a single HTS campaign, even small variability within the cell populations for screening could easily invalidate an entire campaign. Neurodegenerative assays are particularly challenging because neurons are post-mitotic and cannot be expanded for implementation in HTS. Therefore, HTS for neuroprotective compounds requires a c  ...[more]

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